ABSTRACT
OBJECTIVE:To study the protective effects of Baimai ointment on sciatic nerve injury in rabbit. METHODS:The rabbit sciatic nerve injury model was induced by forceps operation. 180 rabbits were randomly divided into 6 groups,with 30 rab-bits in each group (10 rabbits of 7,14,28 d),including sham operation group (blank matrix of Baimai ointment for external use),model group(same as sham operation group),positive group [ig Mecobalamine tablet solution 1.25×10-4 g/(kg·d)] and Bai-mai low-dose,medium-dose and high-dose groups [Baimai ointment 0.33,0.67 and 1.34 g/(kg·d)for external use],once a day, for consecutive 7,14 and 28 d. Gait instrument was used to test the foot-touch-land force of rabbit;pathology examination was conducted for sciatic nerve resection;immunohistochemistry was used to detect the levels of NOS and NMDA in nerve tissue. RE-SULTS:Baimai ointment can significantly relieve sciatic nerve injury,edema and inflammatory reaction;7,14 and 28 d after med-ication,compared with model group,foot-touch-land force decreased significantly in rabbits with sciatic nerve injury (P<0.01);14,28 d after medication,foot-touch-land force of rabbits increased significantly in positive group,Baimai ointment groups,while the levels of NOS and NMDA decreased in nervous tissue(P<0.05). CONCLUSIONS:Baimai ointment can protect sciatic nerve with function injury in rabbits.
ABSTRACT
Objective To compare and analyze the formula composition based on their herbal nature for wind, cold, and dampness arthralgia. Methods The ancient formulas for wind, cold, and dampness arthralgia were searched and the database was established. The top 30 high-frequency herbs were analyzed with frequency, hypothesis testing and association rules. The nature, taste, and meridian distribution were used as the variable quantities for clustering analysis. Results Totally 338 formulas were collected, including 122 formulas for wind arthralgia, 110 formulas for cold arthralgia, and 106 formulas for dampness arthralgia. There are 21 same herbs among the top 30 high-frequency herbs;Tonic herbs were the highest frequently used, followed by the divergence of cold herbs in wind arthralgia, interior-warming herbs in cold arthralgia, and damp-resolving herbs in dampness arthralgia. The frequently used herbs in each kind of formula compared with other two kinds were:Cinnamomi Cortex, Aconiti Lateralis Radix Praeparata, Angelicae Sinensis Radix, Achyranthis Bidentatae Radix, Notopterygii Rhizome et Radix, Paeoniae Radix and Astrragali Radix in formulas for cold arthralgia, Poria and Atractylodis Macrocephalae Rhizoma in formulas for dampness arthralgia. Three kinds of formulas are given priority to slight warm, followed by warm and neutral. Classification is clear when most of the formulas were clustered into five classes according to their herbal nature in each kind of formula. Conclusion The three kinds of formulas cross each other but with own characteristics. A variety of data mining methods can be used to analyze scientific connotation of therapeutic principle for arthralgia.
ABSTRACT
Objective To explore the effects of Sanchi gel on MMP-1 and TIMP-1 expression in epidural adhesion after laminectomy. Methods Laminectomy model was set up in SD rats. 72 SD model rats were divided randomly into Saline group, Zhanlp group, Carbopol Gel group and Sanchi Gel group, with 18 rats in each group. On 7, 14 and 21 days after the laminectomy, immunohistochemistry (method of S-P) was adopted to detect the expression of TIMP-1 and MMP-1. Results Different treatments had different influence on TIMP-1 and MMP-1 expression in epidural adhesion. The expression of TIMP-1 increased gradually on 14 dand 21 d after operation, and Sanqi Gel group showed weaker expression than the other groups (P＜0.05), but the number of positive cells decreased gradually. As to the expression of MMP-1, there was no difference among each group at the end of the second week (P＞0.05). There was a difference between Sanchi Gel group and the other groups at the end of third week (21 d) (P＜0.05) after operation. Sanchi Gel group showed higher expression of TIMP-1 than the other groups. The number of positive cells sharply decreased from 14 d to 21 d after operation. Conclusion Sanchi Gel has a significant preventive effect on fibrous scar formation after laminectomy. The possible mechanism of preventing epidural adhesion after laminectomy by Sanchi Gel could be its regulating and controlling the expression of TIMP-1 and MMP-1 in epidural tissue.